Zheko Radev


A study was conducted to determine the reaction of honey bees of the species Apis mellifera L. to a substance in the spirohidantoin group. The results up to the first hour in the experimental group of the study showed a convulsion and subsequent death of bees who consumed syrop with the used substance. By the end of twelve hour since beginning of the study, bees in two cages from the experimental group were dead, and by the end of the second day, the bees in the other cages were dead. The results in the control group indicate no deaths in the first two days, where the bees consumed pure food. The bee mortality begins on day three, on the ninth day the bees in three of the control cages died, and on the tenth day the bees from another cage also died. In the other two cages, the life span of the bees was fourteen and twentyfour days.

The study and reported results shows the influence of the active substance over honey bees and their life span. More deep stydies are needed in this area of research, and needs to be done outside laboratory tests, analysis with hives.


honey bees, Apis mellifera, spirohidantoins, pesticides

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Dobreva Ts. 2016. Ekotoksikologichna harakteristika na spirohidantoini spryamo medonosnata pchela (Apis mellifica L.). Diplomna rabota, Agraren Universitet-Plovdiv.

Blacquière T., G. Smagghe, C. Gestel, V. Mommaerts. 2012. Neonicotinoids in bees: a review on concentrations, side-effects and risk assessment. Ecotoxicology, 21(4):973–992.

Ganchev D., M. Marinov, R. Prodanova, S. Krustev, M. Zlateva, N. Stoyanov. 2013. In vitro screening for fungicidal activity of cyclopentanespiro-5- hydantoin and its two derivatives towards Alternaria solani and effect on tomato plant varieties. Nauchni trudove na rusenskiya universitet, tom 52, seriya 10(2).

Ganchev D., D. Atanasova, P. Marinova, N. Stoyanov, R. Prodanova, M. Marinov. 2014. Chemical control of the greenhouse whitefly, Trialeurodes vaporariorum (Westwood) on Pelargonium grandiflorum. Agraren Universitet Plovdiv - Nauchni Trudove / Scientific Works of the Agrarian University - Plovdiv, Vol. 58, 203-209 p.

Marinov M., D. Ganchev, P. Marinova, S. Krustev, N. Atanasova, M. Zlateva, N. Stoyanov. 2012. Synthesis of cyclopentanespiro-5-(2, 4-dithiohydantoin) and in vitro insecticidal activity against Cladius pectinicornis. Bulgarian Journal of Agricultural Science, 18(6): 929-933.

Marinov M., D. Ganchev, A. Nikolov, P. Marinova, S. Krustev, V. Madzharova, N. Stoyanov. 2013. In vitro fungicidal activity of cyclopentanespiro-5-hydantoin and its derivatives towards Blumeria graminis f. sp. tritici. Journal Agrarni Nauki, Vol. 4, No. 12, 97-101 p.

Mullin C., M. Frazier, J. Frazier, S. Ashcraft, R. Simonds, D. Engelsdorp, J. Pettis. 2010. High Levels of Miticides and Agrochemicals in North American Apiaries. Implications for Honey Bee Health.

Pimentel D. 1995. Amounts of pesticides reaching target pests: environmental impacts and ethics. Journal of Agriculture and Environmental Ethics, 8:17–29.

Sánchez-Bayo F. 2011. Impacts of Agricultural Pesticides on Terrestrial Ecosystems. In Ecologial Impacts of Toxic Chemicals, 63–87 p.

Smarta L., J. Stevensona. 1982. Laboratory Estimation of Toxicity of Pyrethroid Insecticides to Honeybees: Relevance to Hazard in the Field. Bee World, 63(4):150-152.

Smith T., G. Stratton. 1986. Effects of synthetic pyrethroid insecticides on nontarget organisms. Residue Reviews, Volume 97 of the series Residue Reviews, 93-120 p.

Whitehorn P., S. O’Connor, F. Wackers, D. Goulson. 2012. Neonicotinoid Pesticide Reduces Bumble Bee Colony Growth and Queen Production. Science, 336(6079):351-352.


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